Coding

Part:BBa_K3867005:Design

Designed by: Lei Huang   Group: iGEM21_ICJFLS   (2021-10-13)


APL1 (ADP glucose pyrophosphorylase large subunit 1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1069
    Illegal BglII site found at 1510
    Illegal BamHI site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Two enzymes EcoRI and PstI are used to insert the gene into vector. So these two enzymes site are avoided.


Source

It comes from rDNA of Arabidopsis thaliana.

References